Abstract

Cellular cardiomyoplasty is limited by low levels of engraftment due to cell egress from injection site and death due to anoikis/necrosis.
We hypothesized that tissue-engineered scaffolds that promote rapid cell adhesion would promote transplanted cell engraftment.
We custom-designed stem cell niches, examined encapsulated stem cell viability, proliferation and 18FDG uptake. Acute cardiac retention was measured by cut/count after transplantation of 18FDG labeled rat cardiosphere derived cells (CDCs), in vivo transplanted cell bioenergetics by dual isotope SPECT/CT imaging of Na-Iodide symporter+ CDCs (after 99mTc-pertechnetate administration), and chronic engraftment by bioluminescence imaging (BLI) of luciferase+ CDCs; LV function was assessed by echocardiography.
Crosslinking of N-hydroxysuccinimide-derivatized hyaluronic acid with autologous serum yielded bioadhesive …

Metadata

publication

Circulation 128 (suppl_22), A18277-A18277, 2013

year

2013

publication date

2013/11/26

authors

Angel T Chan, Styliani Vakrou, Kirubel Woldemichael, Junaid Afzal, Andrew Rittenbach, Xiaoping Lin, Sophia Brown, Richard Wahl, Brian O’Rourke, Theodore P Abraham, Benjamin M Tsui, Charles J Steenbergen, Jennifer H Elisseeff, MR Abraham

link

https://www.ahajournals.org/doi/abs/10.1161/circ.128.suppl_22.a18277

source

Circulation

volume

128

issue

suppl_22

pages

A18277-A18277

publisher

Lippincott Williams & Wilkins