Abstract

Rationale:
Molecular imaging is useful for longitudinal assessment of engraftment. However, it is not known which factors, other than cell number, can influence the molecular imaging signal obtained from reporter genes.
Objective:
The effects of cell dissociation/suspension on cellular bioenergetics and the signal obtained by firefly luciferase and human sodium-iodide symporter labeling of cardiosphere-derived cells were investigated.
Methods and Results:
18Fluorodeoxyglucose uptake, ATP levels, 99mTc-pertechnetate uptake, and bioluminescence were measured in vitro in adherent and suspended cardiosphere-derived cells. In vivo dual-isotope single-photon emission computed tomography/computed tomography imaging or bioluminescence imaging (BLI) was performed 1 hour and 24 hours after cardiosphere-derived cell transplantation. Single-photon emission computed tomography quantification was …

Date

February 1, 2013

Authors

Connie Chang, Angel Chan, Xiaoping Lin, Takahiro Higuchi, John Terrovitis, Junaid M Afzal, Andrew Rittenbach, Dongdong Sun, Styliani Vakrou, Kirubel Woldemichael, Brian O’Rourke, Richard Wahl, Martin Pomper, Benjamin Tsui, M Roselle Abraham

Journal

Circulation research

Volume

112

Issue

3

Pages

441-450

Publisher

Lippincott Williams & Wilkins